Sequencing a 903 nucleotide carA gene of <em>Salmonella typhi</em> obtained through PCR employing Ca-3 and Ca-6 primers

Ca(4)IrO(6), Ca(3)MgIrO(6) and Ca(3)ZnIrO(6).

Single crystals of tetracalcium iridium hexaoxide, Ca(4)IrO(6), tricalcium magnesium iridium hexaoxide, Ca(3)MgIrO(6), and tricalcium zinc iridium hexaoxide, Ca(3)ZnIrO(6), were prepared via high-temperature flux growth and structurally characterized by single-crystal X-ray diffraction. The three compounds are isostructural and adopt the K(4)CdCl(6) structure type, comprised of chains of altern...

modulation through activation of PI 3 K / Ca 2 + - independent - and PLC / Ca 2 + - dependent - PKC

We measured pharmacologically isolated GABAergic currents from layer II/III neurons of the rat auditory cortex using patch-clamp recording. Activation of muscarinic receptors by muscarine (1 μM) or oxotremorine (10 μM) decreased the amplitude of electrically evoked inhibitory postsynaptic currents to about one third of their control value. Neither miniature nor exogenously-evoked GABAergic curr...

5'-tailed sequencing primers improve sequencing quality of PCR products.

Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.

Numerous methods have been developed for direct sequencing of single or double-stranded DNA amplified by PCR, and most require template purification steps prior to sequencing to remove excess unincorporated primers and dNTPs. Purification methods can be cumbersome, laborious and time consuming, or yield low DNA recoveries. Strategies for direct sequencing of doublestranded DNA products without ...

بررسی ارزش تشخیصی میزان CA 125, CA 19-9, CA 15-3 مایع پلور در افتراق افیوژن پلورال بدخیم از خوش‌خیم

Background and purpose : Malignant and infectious diseases are the most frequent causes of pleural effusion. Malignant diseases produce pleural effusion through different mechanisms: lymphatic and capillary obstruction, pneumonia or atelectasia. Therefore absence of tumor cells in aspirated effusion, highly declines the sensivity of the cytologic study. For this reason, the use of tumor marke...